Arrangement of Subunits in Peanut Lectin

Salunke, Dinakar M and Swamy, Joginadha M and Khan, Islam M and Mande, Sekhar C and Surolia, Avadhesha and Vijayan, Mamannamana (1985) Arrangement of Subunits in Peanut Lectin. In: The Journal of Biological Chemistry, 260 (25). pp. 13576-13579.

PDF 13576.pdf Restricted to Registered users only Download (1MB) | Request a copy

Abstract

X-ray intensity data from the native orthorhombic crystals of peanut lectin have been collected using oscillation photography. Rotatioq function studies using data up to a resolution of $4.5 \AA$ indicate that the four subunits in the molecule, which constitute the asymmetric unit in the crystals, are related to one another by three mutually perpendicular noncrystallographic 2-fold axes. Chemical cross-linking experiments ins olution followed by sodium dodecyl sulfate gel electrophoresis, carried out in parallel, suggest that thereis more than one type of intersubunit approach in the molecule. Rotation function and cross-linking studies thus show that the tetrameric molecule of pleactniunt is a dimer of a dimer. The two monomers in a dimer are related by 2a- fold axis. The two dimers are in turn related by another 2-fold axis perpendicular to the one that relates the two monomers in the dimer, endowing the inolecule with 222 $(D_2)$ symmetry.

Item Type:	Journal Article
Publication:	The Journal of Biological Chemistry
Publisher:	The American Society of Biological Chemists, Inc.
Additional Information:	Copyright of this article belongs to The American Society of Biological Chemists, Inc.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	10 Jul 2008
Last Modified:	19 Sep 2010 04:47
URI:	http://eprints.iisc.ac.in/id/eprint/14931

Actions (login required)

View Item