X-ray characterisation of an additional binding site in lysozyme

Veerapandian, B and Salunke, DM and Vijayan, M (1985) X-ray characterisation of an additional binding site in lysozyme. In: FEBS Letters, 186 (2). pp. 163-167.

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Abstract

Bromophenol red (BPR) bmds to lysozyme and inhibits. Its activity against bacterial cell walls, but not against the polysaccharide component of peptidoglycan. The binding site of BPR in the enzyme has been characterised by X-ray analysis of the complex at 5 5 \AA resolution. The new binding site, which is outside the cleft close to subsite F, is presumably involved in interactions with the peptide component of peptidoglycan, in the action of lysozyme against bacterial cell walls.

Item Type:	Journal Article
Publication:	FEBS Letters
Publisher:	Elsevier BV
Additional Information:	Copyright of this article belongs to Elsevier BV.
Keywords:	Lysozyme;Bromophenol red;X-ray diffraction;UV absorption;Additional binding site.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	10 Jul 2008
Last Modified:	19 Sep 2010 04:47
URI:	http://eprints.iisc.ac.in/id/eprint/14885

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