Characterization of contryphans from Conus loroisii and Conus amadis that target calcium channels

Sabareesh, V and Gowd, Hanumae K and Ramasamy, P and Sudarslal, S and Krishnan, KS and Sikdar, SK and Balaram, P (2006) Characterization of contryphans from Conus loroisii and Conus amadis that target calcium channels. In: Peptides, 27 (11). pp. 2647-2654.

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Abstract

Distinctly different effects of two closely related contryphans have been demonstrated on voltage-activated $Ca^{2+}$ channels. The peptides Lo959 and Am975 were isolated from Conus loroisii, a vermivorous marine snail and Conus amadis, a molluscivore, respectively. The sequences of Lo959 and Am975 were deduced by mass spectrometric sequencing (MALDI-MS/MS) and confirmed by chemical synthesis. The sequences of Lo959, $GCP^D WDPWC-NH_2$ and Am975, $GCO^D WDPWC-NH_2$ (O: 4-trans-hydroxyproline: Hyp), differ only at residue 3; Pro in Lo959, Hyp in Am975, which is identical to contryphan-P, previously isolated from Conus purpurascens, a piscivore; while Lo959 is a novel peptide. Both Lo959 and Am975 undergo slow conformational interconversion under reverse-phase chromatographic conditions, a characteristic feature of all contryphans reported thus far. Electrophysiological studies performed using dorsal root ganglion neurons reveal that both peptides target high voltage-activated $Ca^{2+}$ channels. While Lo959 increases the $Ca^{2+}$ current, Am975 causes inhibition. The results establish that subtle sequence effects, which accompany post-translational modifications in Conus peptides, can have dramatic effects on target ion channels.

Item Type:	Journal Article
Publication:	Peptides
Publisher:	Elsevier
Additional Information:	Copyright of this article belongs to Elsevier.
Keywords:	Cone snails;Contryphans;Post-translational modifications;Slow conformational interconversion;DRG neurons, Voltage-activated calcium channels;
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	16 May 2008
Last Modified:	23 Jan 2013 09:13
URI:	http://eprints.iisc.ac.in/id/eprint/13988

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