Crystallization and preliminary crystallographic studies on the mannose-specific lectin from garlic

Chandra, Nagasuma R and Dam, Tarun K and Surolia, A and Vijayan, M (1997) Crystallization and preliminary crystallographic studies on the mannose-specific lectin from garlic. In: Acta Crystallographica Section D, 53 . pp. 787-788.

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Abstract

A mannose-specific agglutinin from garlic (Allium sativum which forms part of a well conserved super-family of bulb lectins has been purified and crystallized by the hanging drop vapour-diffusion technique, by equilibrating with a 20%solution of PEG 8000 in the presence of alpha-D-mannose. Crystals of the dimeric form of this protein are monoclinic C2 with unit-cell dimensions a = 203.2, b = 43.8, c = 79.3A, and beta: 112.4 and have two dimers in the asymmetric unit. Data have been collected to 2.4A, resolution and the structure solved by molecular replacement using the coordinates of the snowdrop lectin as the search model.

Item Type:	Journal Article
Publication:	Acta Crystallographica Section D
Publisher:	International Union of Crystallography
Additional Information:	The copyright belongs to International Union of Crystallography
Keywords:	crystallographic studies;lectin;garlic;allium sativum
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	22 Aug 2008
Last Modified:	19 Sep 2010 04:14
URI:	http://eprints.iisc.ac.in/id/eprint/1297

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