Evidence of a trimolecular complex involving LPS, LPS binding protein and soluble CD14 as an effector of LPS response

Thomas, Celestine J and Kapoor, Mili and Sharma, Shilpi and Bausinger, Huguette and Zyilan, Umit and Lipsker, Dan and Hanau, Daniel and Surolia, Avadhesha (2002) Evidence of a trimolecular complex involving LPS, LPS binding protein and soluble CD14 as an effector of LPS response. In: FEBS Letters, 531 (2). pp. 184-188.

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Abstract

The kinetics of the interaction of lipopolysaccharide (LPS), lipopolysaccharide binding protein (LBP) and CD14 was studied using surface plasmon resonance. The association and dissociation rate constants for the binding of LPS and rsCD14 were $2.9 \times 10^4 M^{-1} s^{−1}$ and $0.07 s^{-1}$ respectively, yielding a binding constant of $4.2\times 10^5 M^{-1}$. Significantly, the presence of LBP increased not only the association rate but also the association constant for the interaction between LPS and CD14 by three orders of magnitude. Our experimental results suggest that LBP interacts with LPS and CD14 to form a stable trimolecular complex that has significant functional implications as it allows monocytes to detect the presence of LPS at a concentration as low as 10 pg/ml or 2 pM, and to respond by secreting interleukin-6. Thus, LBP is not merely transferring LPS to CD14 but it forms an integral part of the LPS–rLBP–rsCD14 complex.

Item Type:	Journal Article
Publication:	FEBS Letters
Publisher:	Federation of European Biochemical Societies
Additional Information:	Copyright of this article belongs to Federation of European Biochemical Societies.
Keywords:	Surface plasmon resonance;Lipopolysaccharide;Lipopolysaccharide binding protein;CD14
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	18 Oct 2007
Last Modified:	11 Jan 2013 11:21
URI:	http://eprints.iisc.ac.in/id/eprint/12310

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