MALDI mass sequencing and characterization of filarial glutathione-S-transferase

Gupta, Sarika and Singh, Anchal and Yadav, Marshleen and Singh, Kalyan and Rathaur, Sushma (2007) MALDI mass sequencing and characterization of filarial glutathione-S-transferase. In: Biochemical and Biophysical Research Communications, 356 (2). pp. 381-385.

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Abstract

Glutathione-S-transferase has been detected in the somatic extract and excretory–secretory products of different life stages of Setaria cervi, a bovine filarial parasite. The enzyme was subjected to MALDI-TOF followed by mass spectrometry and the nearest match found was Pleuronectes platessa GST. Molecular mass of the purified enzyme was $\approx 26$ kDa as determined by SDS–PAGE and MALDI-TOF. Setaria cervi GST exhibited high activity towards 1-chloro-2,4-dinitrobenzene and ethacrynic acid. Kinetic analysis with respect to 1-chloro-2,4-dinitrobenzene and glutathione as substrate revealed a $K_m$ of 2.22 mM and 0.61 mM, respectively. The activity was inhibited significantly by Cibacron blue and $\alpha$ -tocopherol.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier
Additional Information:	Copyright of this article belongs to Elsevier.
Keywords:	MALDI mass;Glutathione-S-transferase;Filariasis;Setaria cervi
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	30 Jul 2007
Last Modified:	15 Jan 2013 06:43
URI:	http://eprints.iisc.ac.in/id/eprint/11620

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