The Occurrence of Colistin Resistance in Potential Lactic Acid Bacteria of Food-Producing Animals in India

Ray, M and Ashwini, M and Halami, PM (2024) The Occurrence of Colistin Resistance in Potential Lactic Acid Bacteria of Food-Producing Animals in India. In: Current Microbiology, 81 (9).

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Abstract

The overuse of colistin, the last-resort antibiotic, has led to the emergence of colistin-resistant bacteria, which is a major concern. Lactic acid bacteria which are generally regarded as safe are known to be reservoirs of antibiotic resistance that possibly pose a threat to human and animal health. Therefore, this study assessed the prevalence of colistin antimicrobial resistance in livestock in India, that is lactic acid bacteria in healthy chickens, sheep, beef, and swine of Mysore. Diverse phenotypic and genotypic colistin resistance were examined among the lactic acid bacterial species (n = 84) isolated from chicken (n = 44), sheep (n = 16), beef (n = 14), and swine (n = 10). Hi-comb, double-disk diffusion tests, Minimum Inhibitory Concentration (MIC), and biofilm formation were assessed for phenotypic colistin resistance. Specific primers for colistin-resistant genes were used for the determination of genotypic colistin resistance. Around 20, 18, and 1 were colistin-resistant Lactobacillus, Enterococcus, and Pediococcus species, respectively. Among these, 66.67 exhibited MDR phenotypes, including colistin antibiotic. The identified resistant isolates are Levilactobacillus brevis LBA and LBB (2), Limosilactobacillus fermentum LBF (1), and Pediococcus acidilactici CHBI (1). The mcr-1 and mcr-3 genes were detected in Levilactobacillus brevis LBA, LBB, and Pediococcus acidilactici CHBI isolated from chicken and sheep intestines respectively. The study identified colistin resistance determinants in lactobacilli from food animals, emphasizing the need for enhanced surveillance and monitoring of resistance spread. These findings underscore colistin resistance as a significant medical concern and should be integrated into India�s ongoing antimicrobial resistance monitoring programs. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.

Item Type:	Journal Article
Publication:	Current Microbiology
Publisher:	Springer
Additional Information:	The copyright for this article belongs to the publisher.
Keywords:	bacterial DNA; colistin; lactic acid; antiinfective agent; colistin, animal food; antibiotic resistance; Article; bacterium isolation; biofilm; cell culture; cell suspension; colistin resistance; comb; conjugation; disk diffusion; DNA extraction; Enterococcus; gene mutation; gene sequence; genotype; lactic acid bacterium; Lactobacillus brevis; Lactobacillus fermentum; minimum inhibitory concentration; nonhuman; Pediococcus acidilactici; phenotype; pig; prevalence; sheep; taxonomic identification; animal; antibiotic resistance; bovine; classification; drug effect; Gallus gallus; genetics; India; isolation and purification; Lactobacillales; livestock; microbial sensitivity test; microbiology, Animals; Anti-Bacterial Agents; Cattle; Chickens; Colistin; Drug Resistance, Bacterial; India; Lactobacillales; Livestock; Microbial Sensitivity Tests; Sheep; Swine
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	29 Aug 2024 11:15
Last Modified:	29 Aug 2024 11:15
URI:	http://eprints.iisc.ac.in/id/eprint/86003

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