Singh, S and Pavithra, N and Kaur, H and Varshney, R and Khan, NA and Kumar, R and Sharma, AK and Singh, J and Ramamurthy, PC (2024) Enzyme-based sensor for the real-time detection of atrazine: Evidence from electrochemical and docking studies. In: Scientific Reports, 14 (1).
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Abstract
This study focused on strategically employing the carboxylesterase enzyme Ha006a, derived from the pesticide-resistant microorganism Helicoverpa armigera, to detect atrazine. A comprehensive analysis through biochemical, biophysical and bioinformatics approaches was conducted to determine the interaction between the Ha006a protein and the herbicide atrazine. These experimental findings elucidated the potential of leveraging the inherent pesticide sequestration mechanism of the Ha006a enzyme for sensor fabrication. Numerous optimizations were undertaken to ensure the precision, reproducibility and convenient storage of the resulting electrochemical sensor, Ha006a/MCPE. This biosensor exhibited exceptional performance in detecting atrazine, demonstrating outstanding selectivity with a lower limit of detection of 5.4 µM. The developed biosensor has emerged as a reliable and cost-effective green tool for the detection of atrazine from diverse environmental samples. The Ha006a-based biosensor fabrication has expanded the possibilities for the efficient integration of insect enzymes as analytical tools, paving the way for the design of cost-effective biosensors capable of detecting and quantifying pesticides. © The Author(s) 2024.
| Item Type: | Journal Article |
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| Publication: | Scientific Reports |
| Publisher: | Nature Research |
| Additional Information: | The copyright for this article belongs to the authors. |
| Keywords: | atrazine; carboxylesterase; herbicide, animal; electrochemical analysis; genetic procedures; metabolism; molecular docking; procedures; reproducibility, Animals; Atrazine; Biosensing Techniques; Carboxylesterase; Electrochemical Techniques; Herbicides; Molecular Docking Simulation; Reproducibility of Results |
| Department/Centre: | Division of Interdisciplinary Sciences > Interdisciplinary Centre for Water Research |
| Date Deposited: | 10 Sep 2024 04:28 |
| Last Modified: | 10 Sep 2024 04:28 |
| URI: | http://eprints.iisc.ac.in/id/eprint/85997 |
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