Paper-based nucleic acid sample preparation for point-of-care diagnostics

Soni, S and Toley, BJ (2022) Paper-based nucleic acid sample preparation for point-of-care diagnostics. In: Sensors and Actuators B: Chemical, 355 .

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Abstract

Nucleic acid amplification tests (NAATs) play a crucial role in the early diagnosis and prevention of various infectious diseases. Over the last decade, paper-based devices have garnered attention as low-cost devices for conducting NAATs at the point-of-care (POC). Sample preparation is a critical and one of the most cumbersome steps in a NAAT. We present a comprehensive review of paper-based nucleic acid (NA) sample preparation techniques that have been developed for various complex sample types. We project NAATs as a series of three-unit operations � lysis, purification, and amplification (lysis and purification together constitute the process of sample preparation). Any one or all three operations could be conducted in paper, resulting in multiple possible workflows. We categorize all published paper-based NA sample preparation strategies into one of these workflows, thus presenting a new way to rationally categorize the numerous paper-assisted NA sample preparation workflows. Towards the end, we present a perspective on progress in the field and conclude by enlising important gaps in the area to guide future research. © 2021 Elsevier B.V.

Item Type:	Journal Article
Publication:	Sensors and Actuators B: Chemical
Publisher:	Elsevier B.V.
Additional Information:	The copyright for this article belongs to Elsevier B.V.
Keywords:	Biomolecules; Diagnosis; Nucleic acids; Paper, Global health; Low-resource settings; Medical diagnostics; Micropad; Molecular test; Nucleic acid amplification test; Nucleic acids amplification; Point of care diagnostic; Sample preparation; Work-flows, Purification
Department/Centre:	Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering Division of Mechanical Sciences > Chemical Engineering
Date Deposited:	07 Jan 2022 06:08
Last Modified:	07 Jan 2022 06:08
URI:	http://eprints.iisc.ac.in/id/eprint/70910

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