Novel a-tocopherol-ferrocene conjugates for the specific delivery of transgenes in liver cancer cells under high serum conditions

Asfiya, R and Maiti, B and Kamra, M and Karande, AA and Bhattacharya, S (2021) Novel a-tocopherol-ferrocene conjugates for the specific delivery of transgenes in liver cancer cells under high serum conditions. In: Biomaterials Science, 9 (22). pp. 7636-7647.

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Abstract

The delivery of therapeutic genes to a specific organ has drawn significant research attention. Among the pool of various delivery vectors, cationic liposomes (non-viral) are potential candidates for delivering therapeutic genes due to their low immunogenic response. Here, we have developed novel ferrocene-conjugated cationic tocopheryl aggregates as non-viral vectors. These formulations can transfer a reporter gene (pGL3; encoded for luciferase protein) specifically to liver cancer cells (HepG2 and Huh7) instead of non-hepatic cancer cells, such as Caco-2 (human colon carcinoma) and HeLa (cervical cancer) cells. The transfection efficiency (TE) of the optimum liposomal formulation is more significant than commercially available Lipofectamine 2000 (L2K). Notably, it retains its TE under high serum conditions (up to 50 FBS). A coupled effect from conjugated ferrocene and tocopherol in the cationic liposomal formulation might be responsible for the cell-specific delivery and higher serum compatibility. Therefore, the present proposed delivery system may provide a platform for further progress in terms of developing hepatotropic gene delivery systems. © 2021 The Royal Society of Chemistry.

Item Type:	Journal Article
Publication:	Biomaterials Science
Publisher:	Royal Society of Chemistry
Additional Information:	The copyright for this article belongs to Royal Society of Chemistry
Keywords:	Cytology; Diseases; Gene transfer; Genes; Iron compounds; Lanthanum compounds; Liposomes, Cationics; Condition; Delivery vector; Ferrocene conjugates; Ferrocenes; Liposomal formulation; Liver cancer cells; Therapeutic genes; Transfection efficiency; Transgene, Organometallics
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	21 Dec 2021 05:52
Last Modified:	21 Dec 2021 05:52
URI:	http://eprints.iisc.ac.in/id/eprint/70693

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