A complex of DNA gyrase and RNA polymerase fosters transcription in Mycobacterium smegmatis

Gupta, Richa and China, Arnab and Manjunatha, Ujjini H and Ponnanna, NM and Nagaraja, Valakunja (2006) A complex of DNA gyrase and RNA polymerase fosters transcription in Mycobacterium smegmatis. In: Biochemical and Biophysical Research Communications, 343 (4). pp. 1141-1145.

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Abstract

We report here the existence of a complex between RNA polymerase (RNAP) and DNA gyrase in Mycobacterium smegmatis. The interaction between the two enzymes was detected during our attempts to purify DNA gyrase from M. smegmatis. RNAP subunits co-eluted along with DNA gyrase in two diferent affinity chromatography column procedures employed to purify the latter enzyme. A complex containing both the enzymes was isolated through gel filtration chromatography and sucrose density gradient centrifugation of the cell free extracts. The complex exhibited both DNA supercoiling and transcription activities. Reduction in the transcription activity of the complex in the presence of DNA gyrase inhibitor indicates a role for DNA gyrase in stimulating transcription.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier
Additional Information:	Copyright of this article belongs to Elsevier.
Keywords:	Affinity chromatography;DNA gyrase;Mass spectrometry; Mycobacteria;RNA polymerase
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	20 Nov 2007
Last Modified:	19 Sep 2010 04:27
URI:	http://eprints.iisc.ac.in/id/eprint/6929

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