Samanta, S and Biswas, P and Banerjee, A and Bose, A and Siddiqui, N and Nambi, S and Saini, DK and Visweswariah, SS (2020) A universal stress protein in Mycobacterium smegmatis sequesters the cAMP-regulated lysine acyltransferase and is essential for biofilm formation. In: Journal of Biological Chemistry, 295 (6). pp. 1500-1516.
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Abstract
Universal stress proteins (USPs) are present in many bacteria, and their expression is enhanced under various environmental stresses.Wehave previously identified a USP in Mycobacterium smegmatis that is a product of the msmeg₄₂₀₇ gene and is a substrate for a cAMP-regulated protein lysine acyltransferase (KATms; MSMEG5458). Here, we explored the role of this USP (USP4207) in M. smegmatis and found that its gene is present in an operon that also contains genes predicted to encode a putative tripartite tricarboxylate transporter (TTT). Transcription of the TTT-USP4207 operon was induced in the presence of citrate and tartrate, perhaps by the activity of a divergent histidine kinase-response regulator gene pair. A USP4207-deleted strain had rough colony morphology and reduced biofilm formation compared with the WT strain; however, both normal colony morphology and biofilm formation were restored in a δUSP4207δkatms strain. We identified several proteins whose acetylation was lost in the δkatms strain, and whose transcript levels increased in M. smegmatis biofilms along with that of USP4207, suggesting that USP4207 insulates KATms from its other substrates in the cell.Wepropose that USP4207 sequesters KATms from diverse substrates whose activities are down-regulated by acylation but are required for biofilm formation, thus providing a defined role for this USP in mycobacterial physiology and stress responses.
| Item Type: | Journal Article |
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| Publication: | Journal of Biological Chemistry |
| Publisher: | American Society for Biochemistry and Molecular Biology Inc. |
| Additional Information: | Copyright of this article belongs to American Society for Biochemistry and Molecular Biology Inc. |
| Keywords: | Acetylation; Amino acids; Bacteria; Morphology; Plants (botany); Proteins; Substrates; Transcription, Acyltransferase; Biofilm formation; Colony morphology; Histidine kinase; Mycobacterium smegmatis; Response regulators; Stress response; Transcript level, Biofilms |
| Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics |
| Date Deposited: | 09 Mar 2020 09:37 |
| Last Modified: | 09 Mar 2020 09:37 |
| URI: | http://eprints.iisc.ac.in/id/eprint/64639 |
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