Uzma, M and Sunayana, N and Raghavendra, VB and Madhu, CS and Shanmuganathan, R and Brindhadevi, K (2020) Biogenic synthesis of gold nanoparticles using Commiphora wightii and their cytotoxic effects on breast cancer cell line (MCF-7). In: Process Biochemistry, 92 . pp. 269-276.
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Abstract
The current study aimed at developing gold nanoparticles (AuNPs) using the aqueous extract of the medicinal plant Commiphora wightii. The phytosynthesized gold nanoparticles (Cw@AuNPs) were evaluated for their anticancer activity against MCF-7 breast cancer cell model. The formation of AuNPs by Commiphora wightii leaf extract was confirmed by UV�vis spectra where their surface plasmon resonance was found at 533 nm. Further characterization of Cw@AuNPs was done by transmission electron microscopy (TEM), X-ray diffraction (XRD), energy dispersive X-ray (EDX) analysis, and fourier-transform infrared spectroscopy (FTIR) analysis. In vitro anticancer potential of thus obtained AuNPs was evaluated against MCF-7 and where the IC50 was found to be 66.11 μg/mL Further, apoptotic studies were carried out using ethidium bromide dual staining, DNA fragmentation, comet assay, and flow cytometry studies. Results revealed that Cw@AuNPs at higher concentration significantly increased the apoptotic cells when compared to control cells. Cell cycle analysis of MCF-7 cells confirmed the cell cycle arrest at G2/M phase. These results demonstrate that the biosynthesized Cw@AuNPs appear to be promising for therapeutical applications against breast cancer. © 2020 Elsevier Ltd
Item Type: | Journal Article |
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Publication: | Process Biochemistry |
Publisher: | Elsevier Ltd |
Additional Information: | Copyright of this article belongs to Elsevier Ltd |
Keywords: | Cell culture; Cell death; Diseases; Fiber optic sensors; Fourier transform infrared spectroscopy; Gold compounds; High resolution transmission electron microscopy; Metal nanoparticles; Plant extracts; Surface plasmon resonance; Synthesis (chemical), Anticancer activities; Anticancer potentials; Cytotoxic; DNA damages; Energy dispersive x-ray; MCF-7; MCF-7 breast cancer cells; Phytosynthesis, Gold nanoparticles |
Department/Centre: | Division of Biological Sciences > Biochemistry |
Date Deposited: | 26 May 2021 10:49 |
Last Modified: | 26 May 2021 10:49 |
URI: | http://eprints.iisc.ac.in/id/eprint/64548 |
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