Genomic and Functional Characterization of Poultry Escherichia coli From India Revealed Diverse Extended-Spectrum beta-Lactamase-Producing Lineages With Shared Virulence Profiles

Hussain, Arif and Shaik, Sabiha and Ranjan, Amit and Suresh, Arya and Sarker, Nishat and Semmler, Torsten and Wieler, Lothar H. and Alam, Munirul and Watanabe, Haruo and Chakravortty, Dipshikha and Ahmed, Niyaz (2019) Genomic and Functional Characterization of Poultry Escherichia coli From India Revealed Diverse Extended-Spectrum beta-Lactamase-Producing Lineages With Shared Virulence Profiles. In: FRONTIERS IN MICROBIOLOGY, 10 .

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Abstract

Extended-spectrum beta-lactamases (ESBLs) form the most important resistance determinants prevalent worldwide. Data on ESBL-producing Escherichia coli from poultry and livestock are scarce in India. We present data on the functional and genomic characterization of ESBL-producing E. coli obtained from poultry in India. The whole genome sequences of 28 ESBL-producing E. coli were analyzed comprising of 12 broiler chicken E. coli isolates, 11 free-range chicken E. coli isolates, and 5 human extraintestinal pathogenic E. coli. All of the 28 ESBL-producing E. coli isolates were tested for antibiotic susceptibilities, in vitro conjugation, and virulence-associated phenotypic characteristics. A total of 13 sequence types were identified from the poultry E. coli, which included globally successful sequence types such as ST117 (9%), ST131 (4.3%), and ST10 (4.3%). The most common ESBL gene detected in poultry E. coli genomes was bla(CTX-M-15) (17%). Also, FIB (73%) and FII (73%) were the most common plasmid replicons identified. Conjugation experiments demonstrated 54 (7/13), 30 (3/10), and 40% (2/5) of broiler, free-range, and human ExPEC E. coli to be able to transfer their ESBL genes, respectively. The in vitro virulence-associated phenotypic tests revealed the broiler, free-range, and human ExPEC isolates to be comparable in biofilm formation, resistance to serum bactericidal activity, adherence, and invasion capabilities. Our overall results showed prevalence of virulence phenotypes among the diverse ESBL-producing E. coli from poultry; while certain E. coli clones from broiler-poultry may indeed have the potential to cause infection in humans.

Item Type:	Journal Article
Publication:	FRONTIERS IN MICROBIOLOGY
Publisher:	FRONTIERS MEDIA SA
Additional Information:	Copyright of this article belongs to FRONTIERS MEDIA SA, AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND
Keywords:	poultry Escherichia coli; genomics; ESBL; ExPEC; India
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	06 Jan 2020 10:11
Last Modified:	06 Jan 2020 10:11
URI:	http://eprints.iisc.ac.in/id/eprint/64294

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