Synthetic Arabinomannan Heptasaccharide Glycolipids Inhibit Biofilm Growth and Augment Isoniazid Effects in Mycobacterium smegmatis

Maiti, Krishnagopal and Syal, Kirtimaan and Chatterji, Dipankar and Jayaraman, Narayanaswamy (2017) Synthetic Arabinomannan Heptasaccharide Glycolipids Inhibit Biofilm Growth and Augment Isoniazid Effects in Mycobacterium smegmatis. In: CHEMBIOCHEM, 18 (19). pp. 1959-1970.

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Abstract

Biofilm formation, involving attachment to an adherent surface, is a critical survival strategy of mycobacterial colonies in hostile environmental conditions. Here we report the synthesis of heptasaccharide glycolipids based on mannopyranoside units anchored on to a branched arabinofuranoside core. Two types of glycolipids2,3-branched and 2,5-branchedwere synthesized and evaluated for their efficacies in inhibiting biofilm growth by the non-pathogenic mycobacterium variant Mycobacterium smegmatis. Biofilm formation was inhibited at a minimum biofilm growth inhibition concentration (MBIC) of 100gmL(-1) in the case of the 2,5-branched heptasaccharide glycolipid. Further, we were able to ascertain that a combination of the drug isoniazid with the branched heptasaccharide glycolipid (50gmL(-1)) potentiates the drug, making it three times more effective, with an improved MBIC of 30gmL(-1). These studies establish that synthetic glycolipids not only act as inhibitors of biofilm growth, but also provide a synergistic effect when combined with significantly lowered concentrations of isoniazid to disrupt the biofilm structures of the mycobacteria.

Item Type:	Journal Article
Publication:	CHEMBIOCHEM
Additional Information:	Copy right for this article belongs to the WILEY-V C H VERLAG GMBH, POSTFACH 101161, 69451 WEINHEIM, GERMANY
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	30 Oct 2017 03:40
Last Modified:	30 Oct 2017 03:40
URI:	http://eprints.iisc.ac.in/id/eprint/58094

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