Srivastava, Shireesh and Madras, Giridhar (2001) Hydrolysis of p-nitrophenyl laurate in supercritical carbon dioxide: comparison of two different enzymes. In: Journal of Chemical Technology & Biotechnology, 76 (8). pp. 890-892.
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Abstract
The enzymatic hydrolysis of p-nitrophenyl laurate (PNPL) to p-nitrophenol (PNP) was studied in supercritical carbon dioxide using crude Hog Pancreas Lipase (HPL) and P roqueforti Lipase (PRL). The two enzymes were compared for their optimal activity temperatures and reaction kinetics. HPL had a higher optimal temperature $(65^oC)$ and better yield (22.5%) compared with PRL whose optimal temperature was $50^oC$ with a 15.3% yield. This is considerably higher than the optimum temperature of $37^oC$ and yields of less than 1% observed in the aqueous medium.
Item Type: | Journal Article |
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Publication: | Journal of Chemical Technology & Biotechnology |
Publisher: | Society of Chemical Industry |
Additional Information: | The copyright belongs to Society of Chemical Industry |
Keywords: | enzyme catalysed hydrolysis;Hog pancreas lipase;P roqueforti;Supercritical carbon dioxide |
Department/Centre: | Division of Mechanical Sciences > Chemical Engineering |
Date Deposited: | 20 Mar 2006 |
Last Modified: | 19 Sep 2010 04:24 |
URI: | http://eprints.iisc.ac.in/id/eprint/5610 |
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