A fluorescent molecularly-imprinted polymer gate with temperature and pH as inputs for detection of alpha-fetoprotein

Karfa, Paramita and Roy, Ekta and Patra, Santanu and Kumar, Deepak and Madhuri, Rashmi and Sharma, Prashant K (2016) A fluorescent molecularly-imprinted polymer gate with temperature and pH as inputs for detection of alpha-fetoprotein. In: BIOSENSORS & BIOELECTRONICS, 78 . pp. 454-463.

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Abstract

In this work, we have reported a new approach on the use of stimuli-responsive molecularly imprinted polymer (MIP) for trace level sensing of alpha-fetoprotein (AFP), which is a well know cancer biomarker. The stimuli-responsive MIP is composed of three components, a thermo-responsive monomer, a pH responsive component (tyrosine derivative) and a highly fluorescent vinyl silane modified carbon dot. The synthesized AFP-imprinted polymer possesses excellent selectivity towards their template molecule and dual-stimuli responsive behavior. Along with this, the imprinted polymer was also explored as `OR' logic gate with two stimuli (pH and temperature) as inputs. However, the non-imprinted polymers did not have such `OR' gate property, which confirms the role of template binding. The imprinted polymer was also used for estimation of AFP in the concentration range of 3.96-80.0 ng mL(-1), with limit of detection (LOD) 0.42 ng mL(-1). The role of proposed sensor was successfully exploited for analysis of AFP in real human blood plasma, serum and urine sample. (C) 2015 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	BIOSENSORS & BIOELECTRONICS
Publisher:	ELSEVIER ADVANCED TECHNOLOGY
Additional Information:	Copy right for this article belongs to the ELSEVIER ADVANCED TECHNOLOGY, OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
Keywords:	Molecular gate; Stimuli-responsive polymer; Fluorescence sensor; Molecularly imprinted polymer; Alpha-fetoprotein
Department/Centre:	Division of Chemical Sciences > Solid State & Structural Chemistry Unit
Date Deposited:	29 Feb 2016 06:46
Last Modified:	29 Feb 2016 06:46
URI:	http://eprints.iisc.ac.in/id/eprint/53342

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