Synthesis of cadmium oxide and carbon nanotube based nanocomposites and their use as a sensing interface for xanthine detection

Jain, U and Narang, J and Rani, K and Burna, B and Sunny, S and Chauhan, N (2015) Synthesis of cadmium oxide and carbon nanotube based nanocomposites and their use as a sensing interface for xanthine detection. In: RSC ADVANCES, 5 (38). pp. 29675-29683.

PDF rac_adv-5_28_29675_2015.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

Xanthine oxidase (XOD) extracted from bovine milk was immobilized covalently via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxy succinimide (NHS) chemistry onto cadmium oxide nanoparticles (CdO)/carboxylated multiwalled carbon nanotube (c-MWCNT) composite film electrodeposited on the surface of an Au electrode. The nanocomposite modified Au electrode was characterized by Fourier transform infrared (FTIR), cyclic voltammetry (CV), scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS) before and after immobilization of XOD. Under optimal operation conditions (25 degrees C, + 0.2 V vs. Ag/AgCl, sodium phosphate buffer, pH 7.5), the following characteristics are attributed to the biosensor: linearity of response up to xanthine concentrations of 120 mu M, detection limit of 0.05 mu M (S/N = 3) and a response time of at most 4 s. After being used 100 times over a period of 120 days, only 50% loss of the initial activity of the biosensor was evaluated when stored at 4 degrees C. The fabricated biosensor was successfully employed for the determination of xanthine in fish meat.

Item Type:	Journal Article
Publication:	RSC ADVANCES
Publisher:	ROYAL SOC CHEMISTRY
Additional Information:	Copy right for this article belongs to the ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
Keywords:	DIRECT ELECTRON-TRANSFER; MODIFIED GOLD ELECTRODE; AMPEROMETRIC BIOSENSOR; NANOPARTICLES; FILM; HYPOXANTHINE
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	22 May 2015 09:22
Last Modified:	22 May 2015 09:22
URI:	http://eprints.iisc.ac.in/id/eprint/51588

Actions (login required)

View Item