Sensitive detection of C-reactive protein using optical fiber Bragg gratings

Sridevi, S and Vasu, KS and Asokan, S and Sood, AK (2015) Sensitive detection of C-reactive protein using optical fiber Bragg gratings. In: BIOSENSORS & BIOELECTRONICS, 65 . pp. 251-256.

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Abstract

An accurate and highly sensitive sensor platform has been demonstrated for the detection of C-reactive protein (CRP) using optical fiber Bragg gratings (FBGs). The CRP detection has been carried out by monitoring the shift in Bragg wavelength (Delta lambda(B)) of an etched FBG (eFBG) coated with an anti-CRP antibody (aCRP)-graphene oxide (GO) complex. The complex is characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and atomic force microscopy. A limit of detection of 0.01 mg/L has been achieved with a linear range of detection from 0.01 mg/L to 100 mg/L which includes clinical range of CRP. The eFBG sensor coated with only aCRP (without GO) show much less sensitivity than that of aCRP-GO complex coated eFBG. The eFBG sensors show high specificity to CRP even in the presence of other interfering factors such as urea, creatinine and glucose. The affinity constant of similar to 1.1 x 10(10) M-1 has been extracted from the data of normalized shift (Delta lambda(B)/lambda(B)) as a function of CRP concentration. (C) 2014 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	BIOSENSORS & BIOELECTRONICS
Publisher:	ELSEVIER ADVANCED TECHNOLOGY
Additional Information:	Copy right for this article belongs to the ELSEVIER ADVANCED TECHNOLOGY, OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
Keywords:	C-reactive protein; Fiber Bragg gratings; Graphene oxide; Bragg wavelength
Department/Centre:	Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics Division of Physical & Mathematical Sciences > Physics
Date Deposited:	04 Mar 2015 12:41
Last Modified:	04 Mar 2015 12:41
URI:	http://eprints.iisc.ac.in/id/eprint/50985

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