Inhibition of ERK and proliferation in NK cell lines by soluble HLA-E released from Japanese encephalitis virus infected cells

Shwetank, Shwetank and Date, Onkar Sanjay and Carbone, Ennio and Manjunath, Ramanathapuram (2014) Inhibition of ERK and proliferation in NK cell lines by soluble HLA-E released from Japanese encephalitis virus infected cells. In: IMMUNOLOGY LETTERS, 162 (1, A). pp. 94-100.

PDF imm_let_162-1_94_2014.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

Productive infection of human endothelial cells with Japanese encephalitis virus (JEV), a single stranded RNA virus induces shedding of sHLA-E. We show here that sHLA-E that is released upon infection with this flavivirus can inhibit IL-2 and PMA mediated ERK 1/2 phosphorylation in two NK cell lines, Nishi and NKL. Virus infected or IFN-gamma treated cell culture supernatants containing sHLA-E were found to partially inhibit IL-2 mediated induction of CD25 molecules on NKL cells. It was also found that sHLA-E could inhibit IL-2 induced H-3]-thymidine incorporation suggesting that, similar to cell surface expressed HLA-E, sHLA-E could also inhibit NK cell responses. Hence JEV-induced shedding of sHLA-E needs further investigation to better understand immune responses in JEV infections since it may have a role in viral evasion of NK cell responses. (C) 2014 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	IMMUNOLOGY LETTERS
Publisher:	ELSEVIER SCIENCE BV
Additional Information:	Copy right for this article belongs to the ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
Keywords:	JEV; sHLA-E; NK; Proliferation; ERK; Endothelial cells
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	24 Feb 2015 05:52
Last Modified:	24 Feb 2015 05:52
URI:	http://eprints.iisc.ac.in/id/eprint/50859

Actions (login required)

View Item