Babu, Nagesh and Jyothi, Manchaladore N and Shivaram, Usha and Narayanaswamy, Sharadamma and Rai, Durg Vijay and Devaraj, Varadahalli R (2014) Identification of miRNAs from French bean (Phaseolus vulgaris) under low nitrate stress. In: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, 39 (1). 1-U19.
Full text not available from this repository. (Request a copy)Abstract
Objective: In this study, we report the role of miRNAs involved under nitrogen starvation from widely grown vegetable crop, French bean. In recent years, a great deal of attention has been paid to the elucidation of miRNAs involved in low nitrate stress. Methods: To identify miRNAs expressed under stress, cDNA libraries were analyzed. Results: We reported the nine potential miRNAs with 67 targets involved in nutrient transporters and other stress specific genes. Among the miRNA sequences obtained 6 sequences belong to miR172 family, one with miR169. RT-PCR analysis of expression of miR172 family was induced upon low nitrate stress while miR169 family was repressed. In addition, Pvu-SN7b and Pvu-miR16 may be new members of miRNA172 and miR169 families, respectively. Conclusion: The targets of Pvu-SN7b were major protein kinases, one among which is the Protein Kinase CK2. CK2 Kinase is found to involve in transcription-directed signaling, gene control and cell-cycle regulation. Other targets of Pvu-SN7b were involved in DNA-dependent transcription regulation, photo-periodism, calcium-mediated signaling. Pvu-miR16 targets Thymidine kinase, the key enzyme of deoxy-nucleotide synthesis. The cleavage of these targets affects cell proliferation there by affecting nodule formation. Pvu-miR8 inhibits translation of its target protein Pre-protein translocase, a membrane-bound protein transporter involved in trans-membrane protein transportation. Together these results denote the response and role of miRNAs to nitrate-limiting conditions in French bean.
| Item Type: | Journal Article |
|---|---|
| Publication: | TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI |
| Publisher: | TURKISH BIOCHEM SOC |
| Additional Information: | Copyright for this article belongs to the TURKISH BIOCHEM SOC, HIRFANLI SOKAK BANU, APT 9-3 GAZIOSMANPASA, ANKARA, 06700, TURKEY |
| Keywords: | French bean; low nitrate; protein kinase; photo-periodism |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 11 Jun 2014 10:33 |
| Last Modified: | 11 Jun 2014 10:33 |
| URI: | http://eprints.iisc.ac.in/id/eprint/49200 |
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