Photocytotoxicity of copper(II) complexes of curcumin and N-ferrocenylmethyl-L-amino acids

Goswami, Tridib K and Gadadhar, Sudarshan and Gole, Bappaditya and Karande, Anjali A and Chakravarty, Akhil R (2013) Photocytotoxicity of copper(II) complexes of curcumin and N-ferrocenylmethyl-L-amino acids. In: EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 63 . pp. 800-810.

PDF Eur_Jou_Med_Che_60_800_2013.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

Copper(II) complexes Cu(Fc-aa)(cur)] (1-3) of curcumin (Hcur) and N-ferrocenylmethyl-L-amino acids (Fc-aa), viz., ferrocenylmethyl-L-tyrosine (Fc-TyrH), ferrocenylmethyl-L-tryptophan (Fc-TrpH) and ferrocenylmethyl-L-methionine (Fc-MetH), were prepared and characterized. The DNA photocleavage activity, photocytotoxicity and cellular localization in HeLa and MCF-7 cancer cells of these complexes were studied. Acetylacetonate (acac) complexes Cu(Fc-aa)(acac)] (4-6) were prepared and used as controls. The chemical nuclease inactive complexes showed efficient pUC19 DNA cleavage activity in visible light. Complexes 1-3 showed high photocytotoxicity with low dark toxicity thus giving remarkable photodynamic effect. FACScan analysis showed apoptosis of the cancer cells. Fluorescence microscopic studies revealed primarily cytosolic localization of the complexes. (C) 2013 Elsevier Masson SAS. All rights reserved.

Item Type:	Journal Article
Publication:	EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
Publisher:	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Additional Information:	Copyright for this article belongs to Science Direct
Keywords:	Curcumin; Ferrocene; Copper; L-Amino acids; Photocytotoxicity; Cellular uptake
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	22 Oct 2013 11:21
Last Modified:	22 Oct 2013 11:21
URI:	http://eprints.iisc.ac.in/id/eprint/47529

Actions (login required)

View Item