Antioxidant and oxidative stress parameters in brain of Heteropneustes fossilis under air exposure condition; role of mitochondrial electron transport chain

Paital, Biswaranjan (2013) Antioxidant and oxidative stress parameters in brain of Heteropneustes fossilis under air exposure condition; role of mitochondrial electron transport chain. In: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 95 (1). pp. 69-77.

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Abstract

Many fishes are exposed to air in their natural habitat or during their commercial handling. In natural habitat or during commercial handling, the cat fish Heteropneustes fossilis is exposed to air for > 24 h. Data on its oxidative metabolism in the above condition are not available. Oxidative stress (OS) indices (lipid and protein oxidation), toxic reactive oxygen species (ROS: H2O2) generation, antioxidative status (levels of superoxide dismutase, catalase, glutathione peroxidase and reductase, ascorbic acid and nonprotein sulfhydryl) and activities of electron transport chain (ETC) enzymes (complex I-IV) were investigated in brain tissue of H. fossilis under air exposure condition (0, 3, 6, 12 and 18 h at 25 degrees C). Decreased activities of antioxidant (except catalase) and ETC enzymes (except complex II) with increased H2O2 and OS levels were observed in the tissue under water deprivation condition. Positive correlation was observed for complex II activity and non-protein thiol groups with time period of air exposure. The critical time period to induce OS and to reduce most of the studied antioxidant level in brain was found to be 3-6 h air exposure. The data can be useful to minimize the stress generated during commercial handling of the live fishes those exposed to air in general and H. fossilis in particular. (C) 2013 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Publication:	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Additional Information:	Copyright of this article is belongs to ACADEMIC PRESS INC ELSEVIER SCIENCE
Keywords:	Air exposure; Brain; Electron transport chain; Heteropneustes fossilis; Oxidative stress; Reactive oxygen species toxicity
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	30 Sep 2013 04:52
Last Modified:	30 Sep 2013 04:52
URI:	http://eprints.iisc.ac.in/id/eprint/47339

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