Determination of electric field at and near the focus of a cylindrical lens for applications in fluorescence microscopy

Purnapatra, Subhajit B and Mondal, Partha P (2013) Determination of electric field at and near the focus of a cylindrical lens for applications in fluorescence microscopy. In: AIP ADVANCES, 3 (5).

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Abstract

We present an explicit computable integral solution of the electric field generated at the focal region of a cylindrical lens. This representation is based on vectorial diffraction theory and further enables the computation of the system point spread function of a cylindrical lens. It is assumed that there is no back-scattering and the contribution from the evanescent field is negligible. Stationary phase approximation along with the Fresnel transmission coefficients are employed for evaluating the polarization dependent electric field components. Studies were carried out to determine the polarization effects and to calculate the system resolution. The effect of s -, p - and randomly polarized light is studied on the fixed sample (electric dipole is fixed in space). Proposed approach allows better understanding of electric field effects at the focus of a cylindrical aplanatic system. This opens up future developments in the field of fluorescence microscopy and optical imaging. (C) 2013 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.

Item Type:	Journal Article
Publication:	AIP ADVANCES
Publisher:	AMER INST PHYSICS
Additional Information:	Copyright of this article is belongs AMER INST PHYSICS
Department/Centre:	Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	09 Sep 2013 10:10
Last Modified:	09 Sep 2013 10:10
URI:	http://eprints.iisc.ac.in/id/eprint/47075

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