Active-contour-based automated image quantitation techniques for Western Blot analysis

Pediredla, Adithya Kumar and Seelamantula, Chandra Sekhar (2011) Active-contour-based automated image quantitation techniques for Western Blot analysis. In: 2011 7th International Symposium on Image and Signal Processing and Analysis (ISPA), 4-6 Sept. 2011, Dubrovnik, Croatia.

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Abstract

Western Blot analysis is an analytical technique used in Molecular Biology, Biochemistry, Immunogenetics and other Molecular Biology studies to separate proteins by electrophoresis. The procedure results in images containing nearly rectangular-shaped blots. In this paper, we address the problem of quantitation of the blots using automated image processing techniques. We formulate a special active contour (or snake) called Oblong, which locks on to rectangular shaped objects. Oblongs depend on five free parameters, which is also the minimum number of parameters required for a unique characterization. Unlike many snake formulations, Oblongs do not require explicit gradient computations and therefore the optimization is carried out fast. The performance of Oblongs is assessed on synthesized data and Western Blot Analysis images.

Item Type:	Conference Paper
Publisher:	IEEE
Additional Information:	Copyright of this article belongs to IEEE.
Department/Centre:	Division of Electrical Sciences > Electrical Engineering
Date Deposited:	17 Apr 2013 06:06
Last Modified:	17 Apr 2013 06:06
URI:	http://eprints.iisc.ac.in/id/eprint/46222

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