Unzipping and binding of small interfering RNA with single walled carbon nanotube: A platform for small interfering RNA delivery

Santosh, Mogurampelly and Panigrahi, Swati and Bhattacharyya, Dhananjay and Sood, AK and Maiti, Prabal K (2012) Unzipping and binding of small interfering RNA with single walled carbon nanotube: A platform for small interfering RNA delivery. In: Journal of Chemical Physics, The, 136 (6).

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Abstract

In an effort to design efficient platform for siRNA delivery, we combine all atom classical and quantum simulations to study the binding of small interfering RNA (siRNA) by pristine single wall carbon nanotube (SWCNT). Our results show that siRNA strongly binds to SWCNT surface via unzipping its base-pairs and the propensity of unzipping increases with the increase in the diameter of the SWCNTs. The unzipping and subsequent wrapping events are initiated and driven by van der Waals interactions between the aromatic rings of siRNA nucleobases and the SWCNT surface. However, molecular dynamics (MD) simulations of double strand DNA (dsDNA) of the same sequence show that the dsDNA undergoes much less unzipping and wrapping on the SWCNT in the simulation time scale of 70 ns. This interesting difference is due to smaller interaction energy of thymidine of dsDNA with the SWCNT compared to that of uridine of siRNA, as calculated by dispersion corrected density functional theory (DFT) methods. After the optimal binding of siRNA to SWCNT, the complex is very stable which serves as one of the major mechanisms of siRNA delivery for biomedical applications. Since siRNA has to undergo unwinding process with the effect of RNA-induced silencing complex, our proposed delivery mechanism by SWCNT possesses potential advantages in achieving RNA interference. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.3682780]

Item Type:	Journal Article
Publication:	Journal of Chemical Physics, The
Publisher:	American Institute of Physics
Additional Information:	Copyright of this article belongs to American Institute of Physics.
Department/Centre:	Division of Physical & Mathematical Sciences > Physics
Date Deposited:	10 Apr 2012 07:38
Last Modified:	16 Oct 2018 09:30
URI:	http://eprints.iisc.ac.in/id/eprint/44169

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