Antigen detection assay with parasite specific monoclonal antibodies for diagnosis of lymphatic filariasis

Pandey, Vivek and Madhumathi, Jayaprakasam and Karande, Anjali A and Kaliraj, Perumal (2011) Antigen detection assay with parasite specific monoclonal antibodies for diagnosis of lymphatic filariasis. In: Clinica Chimica Acta, 412 (19-20). pp. 1867-1873.

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Abstract

Background: Lymphatic filariasis is a painful and profoundly disfiguring disease. Infection is usually acquired in childhood but its visible manifestations occur later in life, causing temporary or permanent disability. The importance of developing effective assays to diagnose, monitor and evaluate human lymphatic filariasis has been emphasized by the WHO. Methods: High-affinity monoclonal antibodies (mAbs) specific for recombinant filarial antigen WbSXP-1 were developed. An ELISA based capture assay using monoclonal and polyclonal antibodies for WbSXP-1 was used for detection of circulating filarial antigen. Results: High-affinity monoclonal antibodies (mAbs) were developed that specifically binds both W. bancrofti and B. malayi mf antigens. Two mAbs (1F6H3 and 2E12E3) of subclass IgG2a and IgM showed high affinity, avidity and reactivity to recombinant and mf native antigen. Both the mAbs were used in combination as capture antibodies and polyclonal as detection antibody to develop the assay. The assay showed very high sensitivity towards W. bancrofti mf positive samples compared to endemic normal samples (P<0.0001). Conclusion: A capture assay using high-affinity monoclonal antibodies for WbSXP-1 was developed for the detection of filarial circulating antigen in clinical samples from bancroftian infection. Besides, this would also help in epidemiological studies in endemic areas of filarial infections. (C) 2011 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	Clinica Chimica Acta
Publisher:	Elsevier Science B.V.
Additional Information:	Copyright of this article belongs to Elsevier Science B.V.
Keywords:	Lymphatic filariasis;SXP-1;Monoclonal antibodies; Microfilaria;Antigen detection assay
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	20 Sep 2011 08:29
Last Modified:	20 Sep 2011 08:29
URI:	http://eprints.iisc.ac.in/id/eprint/40542

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