Subramanian, N and Adiga, PR (1995) Simultaneous purification of biotin-binding proteins-I and -II from chicken egg yolk and their characterization. In: Biochemical Journal, 308 (part 2). pp. 573-577.
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Abstract
Chicken egg yolk biotin-binding protein-I (BBP-I) has been purified to homogeneity along with the tetrameric BBP-II by a common protocol. The purification includes delipidation of egg yolk by butanol extraction, DEAE-Sephacel chromatography, treatment with guanidinium chloride and biotin-aminohexyl-Sepharose affinity chromatography. The identity of purified BBP-I was ascertained by its physicochemical properties as well as by its immunological cross-reactivity and precursor-product relationship with BBP-II.
Item Type: | Journal Article |
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Publication: | Biochemical Journal |
Publisher: | Portland Press |
Additional Information: | Copyright of this article belongs to Portland Press. |
Department/Centre: | Division of Biological Sciences > Biochemistry Division of Biological Sciences > Molecular Reproduction, Development & Genetics |
Date Deposited: | 26 May 2011 07:29 |
Last Modified: | 02 Nov 2011 07:48 |
URI: | http://eprints.iisc.ac.in/id/eprint/37898 |
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