Selvaraj, N and Moudgal , NR (1994) In vivo and in vitro studies on the differential role of luteinizing hormone and follicle-stimulating hormone in regulating follicular function in the bonnet monkey (Macaca radiata) using specific gonadotropin antibodies. In: Biology of Reproduction, 51 (2). 246-253 .
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Abstract
Although a distinct need for FSH in the regulation of follicular maturation in the primate is well recognized, it is not clear how FSH controls the functionality of different cellular compartments of the follicle. It is also not evident whether there is a requirement for LH in follicular maturation in the primate. In the first part of the present study, female bonnet monkeys were administered a well-characterized ovine (o) LH antiserum to neutralize endogenous monkey LH for different periods during the follicular phase, and the effect on the overall follicular maturation process was assessed by analyzing serum estrogen (E) and progesterone (P) profiles. Neither continuous LH deprivation from Day 8 of the cycle nor deprivation of LH on any one day between Days 6 and 10 had a significant effect on serum E and P profiles and the follicular maturation process. The period for which the antiserum was effective was dependent upon the dose injected; 1 ml of the antiserum given on Day 8 blocked ovulation but not follicular maturation. To assess the effect of deprivation of LH/FSH at the cellular level, animals were deprived in vivo of LH (on Days 8 and 9 of the cycle) or of FSH (on Day 6 of the cycle) by injection of highly characterized hCG and ovine (o) FSH antisera, respectively; the in vitro responsiveness of granulosa and thecal cells isolated on Day 10 from these animals was then determined.(ABSTRACT TRUNCATED AT 250 WORDS)
Item Type: | Journal Article |
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Publication: | Biology of Reproduction |
Publisher: | Society for the Study of Reproduction |
Additional Information: | Copyright of this article belongs to Society for the Study of Reproduction. |
Department/Centre: | Division of Biological Sciences > Biochemistry Division of Biological Sciences > Molecular Reproduction, Development & Genetics |
Date Deposited: | 20 May 2011 10:42 |
Last Modified: | 02 Nov 2011 05:34 |
URI: | http://eprints.iisc.ac.in/id/eprint/36618 |
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