In vitro and in vivo effects of methyl isocyanate on rat brain mitochondrial respiration

Jeevaratnam, K and Vidya, S (1994) In vitro and in vivo effects of methyl isocyanate on rat brain mitochondrial respiration. In: Archives of Environmental Contamination and Toxicology, 27 (2). 272 -275 .

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Abstract

The present study deals with the in vitro and in vivo effects of methyl isocyanate (MIC) on rat brain mitochondrial function. Addition of MIC to tightly coupled brain mitochondria in vitro resulted in a mild stimulation of state 4 respiration, abolition of respiratory control, decrease in ADP/0 ratio, and inhibition of state 3 oxidation. The oxidation of NAD+-linked substrates (glutamate + malate) was more sensitive (fourfold) to the inhibitory action of MIC than succinate while cytochrome oxidase was unaffected. Administration of MIC subcutaneously at a lethal dose affected respiration only with glutamate + malate as the substrate (site I) and caused a 20% decrease in state 3 oxidation leading to a significant decrease in respiratory control index while state 4 respiration and ADP/O ratio remained unaffected. As both the malondialdehyde and iron contents of brain mitochondria were not altered, it may be inferred that the observed in vivo inhibition of state 3 oxidation is induced by MIC through systemic stagnant hypoxia leading to ischemia of brain, which further contributes to the cerebral hypoxia.

Item Type:	Journal Article
Publication:	Archives of Environmental Contamination and Toxicology
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	12 Apr 2011 05:51
Last Modified:	12 Apr 2011 05:51
URI:	http://eprints.iisc.ac.in/id/eprint/36564

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