Breast cancer and human papillomavirus infection: No evidence of HPV etiology of breast cancer in Indian women

Hedau, Suresh and Kumar, Umesh and Hussain, Showket and Shukla, Shirish and Pande, Shailja and Jain, Neeraj and Tyagi, Abhishek and Deshpande, Trivikram and Bhat, Dilafroze and Mir, Mohammad Muzaffar and Chakraborty, Sekhar and Singh, Mohan Y and Kumar, Rakesh and Somasundaram, Kumaravel and Bharti, Alok C and Das, Bhudev C (2011) Breast cancer and human papillomavirus infection: No evidence of HPV etiology of breast cancer in Indian women. In: BMC Cancer, 11 .

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Abstract

Background: Two clinically relevant high-risk HPV (HR-HPV) types 16 and 18 are etiologically associated with the development of cervical carcinoma and are also reported to be present in many other carcinomas in extra-genital organ sites. Presence of HPV has been reported in breast carcinoma which is the second most common cancer in India and is showing a fast rising trend in urban population. The two early genes E6 and E7 of HPV type 16 have been shown to immortalize breast epithelial cells in vitro, but the role of HPV infection in breast carcinogenesis is highly controversial. Present study has therefore been undertaken to analyze the prevalence of HPV infection in both breast cancer tissues and blood samples from a large number of Indian women with breast cancer from different geographic regions. Methods: The presence of all mucosal HPVs and the most common high-risk HPV types 16 and 18 DNA was detected by two different PCR methods - (i) conventional PCR assays using consensus primers (MY09/11, or GP5 +/GP6+) or HPV16 E6/E7 primers and (ii) highly sensitive Real-Time PCR. A total of 228 biopsies and corresponding 142 blood samples collected prospectively from 252 patients from four different regions of India with significant socio-cultural, ethnic and demographic variations were tested. Results: All biopsies and blood samples of breast cancer patients tested by PCR methods did not show positivity for HPV DNA sequences in conventional PCRs either by MY09/11 or by GP5+/GP6+/HPV16 E6/E7 primers. Further testing of these samples by real time PCR also failed to detect HPV DNA sequences. Conclusions: Lack of detection of HPV DNA either in the tumor or in the blood DNA of breast cancer patients by both conventional and real time PCR does not support a role of genital HPV in the pathogenesis of breast cancer in Indian women.

Item Type:	Journal Article
Publication:	BMC Cancer
Publisher:	Biomed Central
Additional Information:	Copyright of this article belongs to Biomed Central.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	08 Mar 2011 06:59
Last Modified:	08 Mar 2011 06:59
URI:	http://eprints.iisc.ac.in/id/eprint/35910

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