A simplified method for the purification of riboflavin-binding protein from hen's egg yolk

Murthy, Usha S and Sreekrishna, K and Adiga, PR (1979) A simplified method for the purification of riboflavin-binding protein from hen's egg yolk. In: Analytical Biochemistry, 92 (2). pp. 345-350.

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Abstract

A simple and efficient procedure for the purification of the riboflavin-binding protein from hen's egg yolk is described. This method involves the removal by exclusion of lipoproteins and subsequent fractionation of soluble yolk proteins held on a DEAE-cellulose column by a salt gradient which is followed by purification by gel filtration on Sephadex G-100. The protein thus isolated is homogeneous by various physicoehemical, immunological, and functional criteria.

Item Type:	Journal Article
Publication:	Analytical Biochemistry
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	30 Nov 2010 12:16
Last Modified:	30 Nov 2010 12:16
URI:	http://eprints.iisc.ac.in/id/eprint/34083

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