Studies on the macroconida of microsporum-canis-characteristics of invitro amino-acid incorporativing system

Jayaram, BM and Rao, Ramananda G (1979) Studies on the macroconida of microsporum-canis-characteristics of invitro amino-acid incorporativing system. In: Archives of Microbiology, 120 (1). pp. 67-72.

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Abstract

The characteristics of an in vitro polyuridylic acid dependent amino acid incorporating system prepared from germinating macroconidia of Microsporum canis are described. The incorporation of 14C-phenylalanine into polyphenylalanine is dependent on S-30 extract, adenosine triphosphate, magnesium ions and polyuridylic acid. Incorporation is slightly enhanced by yeast transfer ribonucleic acid and pyruvate kinase. The system is highly sensitive to ribonuclease, puromycin and miconazole (an antifungal agent), moderately sensitive to sodium fluoride and much less sensitive to phenethylalcohol, cycloheximide, chloramphenicol and deoxyribonuclease. Cell-free extract from ungerminated conidia has less capacity to synthesize the protein and during germination a marked increase in the protein synthetic activity is observed. The results from experiments wherein ribosomes and S-100 fraction from germinated and ungerminated spores are interchanged, revealed that the defect in the extract from the ungerminated spore is in the ribosomes.

Item Type:	Journal Article
Publication:	Archives of Microbiology
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer .
Keywords:	Microsporum cauls -In vitro protein synthesis - Effect of inhibitors - Limiting factor.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	27 Dec 2010 13:10
Last Modified:	27 Dec 2010 13:10
URI:	http://eprints.iisc.ac.in/id/eprint/33899

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