Vaishnav, YN and Antony, A (1989) Antibodies raised against denatured DNA bind to double-stranded DNA. In: Journal of Immunological Methods, 118 (1). pp. 25-30.
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Antibodies raised against denatured DNA complexed with methylated bovine serum albumin have been reported to react with ssDNA but not with dsDNA. Using a highly sensitive avidin-biotin microELISA, we report that such antibodies also bind to dsDNA. Antibodies which reacted with ssDNA and dsDNA were found to be IgG type. The antibodies did not react with tRNA and rRNA. The binding of antibodies to dsDNA was partially inhibited dy individual deoxyribonucleotides. ssDNA as well as dsDNA inhibited the binding of antibodies to dsDNA. The binding of these antibodies to supercoiled and relaxed forms of pBR322 DNA was demonstrated by gel retardation assay. The cross-reaction with ssDNA was observed even after affinity purification on native DNA-cellulose. The antibodies were also shown to bind to poly(dA-dT)·poly(dA-dT)
| Item Type: | Journal Article |
|---|---|
| Publication: | Journal of Immunological Methods |
| Publisher: | Elsevier science |
| Additional Information: | Copyright of this article belongs to Elsevier science. |
| Keywords: | Anti-DNA antibody;dsDNA-binding;Avidin-biotin microELISA;Gel retardation assay. |
| Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
| Date Deposited: | 27 Aug 2010 06:22 |
| Last Modified: | 27 Aug 2010 06:22 |
| URI: | http://eprints.iisc.ac.in/id/eprint/31557 |
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