Induction of allergen-specific IgE and IgG responses by anti-idiotypic antibodies

Nagpal, S and Shanthi, KN and Kori, R and Schroder, H and Metcalfe, DD and Rao, Subba PV (1989) Induction of allergen-specific IgE and IgG responses by anti-idiotypic antibodies. In: The Journal of Immunology, 142 (10). pp. 3411-3415.

PDF indu.pdf - Published Version Restricted to Registered users only Download (692kB) | Request a copy

Abstract

In order to explore idiotypic, anti-idiotypic, and anti-anti-idiotypic responses to allergens, BALB/c mice were immunized with affinity- purified human idiotypic antibodies directed against a highly purified shrimp allergen. This resulted in the production of anti-idiotypic antibodies which were quantitated by using rabbit idiotypic antibodies raised against the same purified allergen. The mouse anti-idiotypic antibodies recognized shrimp-specific human idiotypic antibodies of the IgE isotype from 18 of 20 individuals, and IgG antibodies from 14 of 20 shrimp-sensitive patients. Immunization of BALB/c mice with affinity- purified, allergen-specific anti-idiotypic antibodies induced anti- allergen IgE and IgG responses in the absence of the allergen. This paper thus presents evidence that anti-idiotypic antibodies raised against allergen-specific idiotypic antibodies may substitute for the original allergen in the induction of allergen-specific idiotypic antibodies. The demonstration of shared idiotopes on IgG and IgE antibodies in the sera of shrimp-sensitive patients supports the use of allergen-specific anti-idiotypic antibodies as surrogate allergens.

Item Type:	Journal Article
Publication:	The Journal of Immunology
Publisher:	American Association of Immunologists
Additional Information:	Copyright of this article belongs to American Association of Immunologists.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	19 Aug 2010 12:56
Last Modified:	19 Sep 2010 06:14
URI:	http://eprints.iisc.ac.in/id/eprint/31448

Actions (login required)

View Item