Detection,amplification and sequence homology of sodC in clinical isolates of Salmonella sp

Sanjay, MK and Shrideshikan, SM and Usha, MS and PhilipRaj, A and Gaddad, SM and Shivannavar, CT (2010) Detection,amplification and sequence homology of sodC in clinical isolates of Salmonella sp. In: Indian Journal of Medical Research, 131 (4). pp. 565-570.

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Abstract

Background & objectives: Periplasmic copper and zinc superoxide dismutase (Cu,Zn-SOD or SodC) is an important component of the antioxidant shield which protects bacteria from the phagocytic oxidative burst. Cu,Zn-SODs protect Gram-negative bacteria against oxygen damage which have also been shown to contribute to the pathogenicity of these bacterial species. We report the presence of SodC in drug resistant Salmonella sp. isolated from patients suffering from enteric fever. Further sodC was amplified, cloned into Escherichia coli and the nucleotide sequence and amino acid sequence homology were compared with the standard strain Salmonella Typhimurium 14028. Methods: Salmonella enterica serovar Typhi (S. Typhi) and Salmonellaenterica serovar Paratyphi (S. Paratyphi) were isolated and identified from blood samples of the patients. The isolates were screened for the presence of Cu, Zn-SOD by PAGE using KCN as inhibitor of Cu,Zn-SOD. The gene (sodC) was amplified by PCR, cloned and sequenced. The nucleotide and amino acid sequences of sodC were compared using CLUSTAL X.Results: SodC was detected in 35 per cent of the Salmonella isolates. Amplification of the genomic DNA of S. Typhi and S. Paratyphi with sodC specific primers resulted in 519 and 515 bp amplicons respectively. Single mutational difference at position 489 was observed between thesodC of S. Typhi and S. Paratyphi while they differed at 6 positions with the sodC of S. Typhimurium 14028. The SodC amino acid sequences of the two isolates were homologous but 3 amino acid difference was observed with that of standard strain S. Typhimurium 14028.Interpretation & conclusions: The presence of SodC in pathogenic bacteria could be a novel candidate as phylogenetic marker.

Item Type:	Journal Article
Publication:	Indian Journal of Medical Research
Publisher:	Indian Council of Medical Research
Additional Information:	Copyright of this article belongs to Indian Council of Medical Research.
Keywords:	Cloning;Cu,Zn-SOD;Gram-negative bacteria;Salmonella; sequence homology; sodC
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	04 Jun 2010 09:48
Last Modified:	19 Sep 2010 06:06
URI:	http://eprints.iisc.ac.in/id/eprint/27716

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