Prevalence and analysis of t(14;18) and t(11;14) chromosomal translocations in healthy Indian population

Nambiar, Mridula and Raghavan, Sathees C (2010) Prevalence and analysis of t(14;18) and t(11;14) chromosomal translocations in healthy Indian population. In: Annals of Hematology, 89 (1). pp. 35-43.

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Abstract

Hematopoietic malignancies like leukemia and lymphoma are characteristically associated with various chromosomal translocations. Follicular lymphoma (FL) and mantle cell lymphoma (MCL) are two subtypes of non-Hodgkin's lymphoma which possess t(14;18) and t(11;14) translocations, respectively. The incidence of FL and MCL is higher in the western countries as compared to India. Interestingly, the associated translocations are also found in healthy individuals in western population, which is 50-80% for t(14;18), whereas t(11;14) occurs at a very low frequency. However, there are no studies to explore thes translocations in healthy Indian population, which could explain the lower incidence of FL and MCL. We employed Southern hybridization following nested PCR to detect above translocations in healthy individuals from India. Our results suggest that this assay can detect one t(14;18) translocation event in up to 10(7) normal cells where as one t(11;14) in 10(8) normal cells. According to our results, 87 out of 253 individuals carry t(14;18) indicating 34% prevalence in the population. The presence of this translocation was also detectable at the transcript level. Although, no gender-based difference was observed, an age-dependent increase in the prevalence of translocation was found in adults. However, even after studying 210 people, we could not detect any t(11;14) translocation, indicating that it is uncommon in Indian population. These results suggest that lower incidence of FL and MCL in India could be attributed to lower prevalence of these translocations in healthy individuals.

Item Type:	Journal Article
Publication:	Annals of Hematology
Publisher:	Springer.
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	Non-Hodgkin's lymphoma;Follicular lymphoma; Mantle cell lymphoma;V(D)J recombination; BCL2/J(H) translocation; BCL1/J(H) translocation.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	01 Jan 2010 08:43
Last Modified:	19 Sep 2010 05:53
URI:	http://eprints.iisc.ac.in/id/eprint/25179

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