Ghosh, Arundhati and Nayak, Rabindranath and Shaila, MS (1996) Inhibition of replication of rinderpest virus by 5-fluorouracil. In: Antiviral Research, 31 (1-2). pp. 35-44.
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Abstract
5-Fluorouracil (5FU), an analogue of uracil, was found to inhibit the production of infectious particles of rinderpest virus (RPV) in Vero cells (African green monkey kidney cells) by 99%, at a concentration of 1 μg/ml. The levels of individual mRNA specific for five of the virus genes were also reduced drastically, while the level of mRNA for a cellular housekeeping gene—glyceraldehyde-3-phosphate dehydrogenase (GAPDH)—was unaltered by fluorouracil treatment of infected cells. Both virus RNA and protein synthesis showed inhibition in a dose-dependent manner. The virions which budded out of 5-fluorouracil-treated cells also contained reduced amounts of virus proteins compared with virus particles from untreated cells.
Item Type: | Journal Article |
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Publication: | Antiviral Research |
Publisher: | Elsevier Science |
Additional Information: | Copyright of this article belongs to Elsevier Science. |
Keywords: | Rinderpest virus;5-Fluorouracil;Virus transcription; Antiviral drug. |
Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
Date Deposited: | 21 Jan 2010 05:32 |
Last Modified: | 19 Sep 2010 05:51 |
URI: | http://eprints.iisc.ac.in/id/eprint/24770 |
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