Plant regeneration from protoplasts of Capsicum annuum L cv. California Wonder

Prakash, AH and Rao, K Sankara and Kumar, M Udaya (1997) Plant regeneration from protoplasts of Capsicum annuum L cv. California Wonder. In: Journal of bioscience, 22 (3). pp. 339-344.

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Abstract

Plant regeneration from mesophyll protoplasts of pepper, Capsicum annuum L. cv. California Wonder has been demonstrated via shoot organogenesis, Protoplasts isolated from fully expanded leaves of 3-week-old axenic shoots when cultured in TM medium supplemented with 1 mgl(-1) NAA, 1 mgl(-1) 2, 4-D, 0.5 mgl(-1) BAP (CM 1) resulted in divisions with a frequency ranging from 20-25%. Antioxidant ascorbic acid and polyvinylpyrrolidone (PVP) in the medium and incubation in the dark helped overcome browning of protoplasts. Microcalli and macrocalli were formed in TM medium containing 2 mgl(-1) NAA and 0.5 mgl(-1) BAP (CM II) and MS gelled medium containing 2 mgl(-1) NAA and 0.5 mgl(-1) BAP (CM III), respectively, Regeneration of plantlets was possible via caulogenesis, Microshoots, 2-5 per callus appeared on MS gelled medium enriched with 0.5 mgl(-1) IAA, 2 mgl(-1) GA and 10 mgl(-1) BAP (CM IVc). Rooting of microshoots was obtained on half strength gelled medium containing 1 mgl(-1) NAA and 0.5 mgl(-1) BAP, Protoplasts isolated from cotyledons failed to divide and degenerated eventually.

Item Type:	Journal Article
Publication:	Journal of bioscience
Publisher:	Indian academy of sciences
Additional Information:	Copyright for this article belongs to Indian academy of sciences.
Keywords:	leaf protoplasts;Capsicum annuum L cv California Wonder; regeneration;organogenesis.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	27 Jan 2010 08:20
Last Modified:	19 Sep 2010 05:51
URI:	http://eprints.iisc.ac.in/id/eprint/24689

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