Vanadate stimulates oxidation of NADH to generate hydrogen peroxide

Vijaya, S and Ramasarm, T (1984) Vanadate stimulates oxidation of NADH to generate hydrogen peroxide. In: Journal of Inorganic Biochemistry, 20 (3). pp. 247-254.

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Abstract

Vanadate in the polymeric form of decavanadate, but not other forms, stimulated oxidation of NADH to NAD+ NADPH was also oxidized with comparable rates. This oxidation of NADH was accompanied by uptake of oxygen and generated hydrogen peroxide with the following stoichiometry: NADH + H+ + O2 → NAD+ + H2O2. The reaction followed second-order kinetics. The rate was dependent on the concentration of both NADH and vanadate and increased with decreasing pH. The reaction had an obligatory requirement for phosphate ions. Esr studies in the presence of the spin trap dimethyl pyrroline N oxide indicated the involvement of Superoxide anion as an intermediate. The reaction was sensitive to Superoxide dismutase and other scavengers of superoxide anions.

Item Type:	Journal Article
Publication:	Journal of Inorganic Biochemistry
Publisher:	Elsevier science
Additional Information:	Copyright for this article belongs to Elsevier science .
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	29 Jan 2010 05:14
Last Modified:	19 Sep 2010 05:47
URI:	http://eprints.iisc.ac.in/id/eprint/23802

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