Requirement for calcium ions in mycobacteriophage I3 DNA injection and propagation

Nagaraja, Valakunja and Gopinathan, KP (1980) Requirement for calcium ions in mycobacteriophage I3 DNA injection and propagation. In: Archives of Microbiology, 124 (2-3). pp. 249-254.

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Abstract

Ca2+ ions are absolutely necessary for the propagation of mycobacteriophage I3 in synthetic medium. These ions are required for successful infection of the host and during the entire span of the intracellular development of the phage. A direct assay of the phage DNA injection using 32[P] labelled phage, showns that Ca2+ ions are necessary for the injection process. The injection itself is a slow process and takes 15 min to complete at 37°C. The bacteria infected in presence of Ca2+ tend to abort if the ions are subsequently withdrawn from the growth medium. The effect of calcium withdrawal is maximally felt during the early part of the latent period; however, later supplementation of Ca2+ ions salvage phage production and the mature phage progeny appear after a delayed interval, proportional to the time of addition of Ca2+.

Item Type:	Journal Article
Publication:	Archives of Microbiology
Publisher:	Springer Berlin / Heidelberg
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	Calcium ions - Phage propagation - Phage DNA injection - Mycobacteriophage 13
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	02 Feb 2010 10:45
Last Modified:	19 Sep 2010 05:43
URI:	http://eprints.iisc.ac.in/id/eprint/22921

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