A Method for Isolation of Protoplasts from Dermatophytes

Srikantha, T and Rao, G Ramananda (1984) A Method for Isolation of Protoplasts from Dermatophytes. In: Journal of General Microbiology, 130 (Jun). pp. 1503-1506.

PDF 7.pdf - Published Version Restricted to Registered users only Download (575kB) | Request a copy

Abstract

A method has been developed to isolate protoplasts from dermatophytes using Novozym 234. A simple technique of flotation in MgSO, has been adapted to separate protoplasts from incubation mixture. Electron microscopic studies confirmed the absence of cell wall material on these protoplasts. The recovery of DNA from protoplasts was higher than from mycelia.

Item Type:	Journal Article
Publication:	Journal of General Microbiology
Publisher:	Society for General Microbiology
Additional Information:	Copyright for this article belongs to Society for General Microbiology.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	28 Jan 2010 06:31
Last Modified:	19 Sep 2010 05:43
URI:	http://eprints.iisc.ac.in/id/eprint/22893

Actions (login required)

View Item