Inhibition of monkey liver serine hydroxymethyltransferase by Cibacron Blue 3G-A

Ramesh, KS and Rao, N Appaji (1980) Inhibition of monkey liver serine hydroxymethyltransferase by Cibacron Blue 3G-A. In: Biochemical Journal, 187 (1). pp. 249-252.

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Abstract

Cibacron Blue 3G-A inhibited monkey liver serine hydroxymethyltransferase competitively with respect to tetrahydrofolate and non-competitively with respect to L-serine. NADH, a positive heterotropic effector, failed to protect the enzymes against inhibition by the dye and was unable to desorb the enzyme from Blue Sepharose CL-6B gel matrix. The binding of the dye to the free enzyme was confirmed by changes in the dye absorption spectrum. The results indicate that the dye probably binds at the tetrahydrofolate-binding domain of the enzyme, rather than at the 'dinucleotide fold'.

Item Type:	Journal Article
Publication:	Biochemical Journal
Publisher:	The Biochemical Society
Additional Information:	Copyright of this article belongs to the Biochemical Society.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	31 Aug 2009 13:11
Last Modified:	27 Dec 2010 08:40
URI:	http://eprints.iisc.ac.in/id/eprint/22700

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