Trehalose Toxicity in Cuscuta reflexa - Sucrose Content Decreases In Shoot Tips Upon Trehalose Feeding

Veluthambi, K and Mahadevan, S and Maheshwari, Ramesh (1982) Trehalose Toxicity in Cuscuta reflexa - Sucrose Content Decreases In Shoot Tips Upon Trehalose Feeding. In: Plant Physiology, 69 (6). pp. 1247-1251.

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Abstract

Trehalose, an alpha,alpha-diglucoside, induced a rapid blackening and death of shoot tips of Cuscuta reflexa (dodder) cultured in vitro. The onset of toxic symptom was delayed if any of the several sugars which support the in vitro growth of Cuscuta was supplied with trehalose. The rate of trehalose uptake or its accumulation in the tissue was not affected by sugar cofeeding. The levels of total and reducing sugars declined appreciably in the trehalose-fed shoot tip explants compared to control tissue cultured in absence of a carbon source. This was not due to an increased rate of respiration of the trehalose-treated tissue. In shoot tips cultured in presence of both trehalose and sucrose, the decline in total and reducing sugars was curtailed. There was a marked fall in the level of sucrose; and invertase activity was higher in trehalose-fed shoot tips. The incorporation of label from [14C]glucose into sucrose in the shoot tip explant was reduced as early as 12 h of trehalose feeding. The results suggest that increased utilization of sucrose as well as an inhibition of its synthesis contribute to the drastic fall in the sucrose content upon trehalose feeding.

Item Type:	Journal Article
Publication:	Plant Physiology
Publisher:	American Society of Plant Biologists
Additional Information:	Copyright of this article belongs to American Society of Plant Biologists.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	29 Dec 2009 10:09
Last Modified:	19 Sep 2010 05:37
URI:	http://eprints.iisc.ac.in/id/eprint/21505

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