Expression Profiling of Nucleotide Metabolism-Related Genes in Human Breast Cancer Cells After Treatment with 5-Fluorouracil

Behera, Rajendra Kumar and Nayak, Rabindranath (2009) Expression Profiling of Nucleotide Metabolism-Related Genes in Human Breast Cancer Cells After Treatment with 5-Fluorouracil. In: Cancer investigation, 27 (5). pp. 561-567.

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Abstract

5-Fluorouracil (5-FU) is one of the most widely used drugs for treatment of cancers, including breast cancer that exhibits its anticancer activity by inhibiting DNA synthesis and also incorporated into DNA and RNA. The objective of this investigation was to find out the total nucleotide metabolism genes regulated by 5-FU in breast cancer cell line. The breast cancer cell line MCF-7 was treated with the drug 5-FU. To analyze the expression of genes, we have conducted the experiment using 1.7k and 19k human microarray slide and confirmed the expression of genes by semiquantitative reverse transcription-polymerase chain reaction. The expression of 44 genes involved in the nucleotide metabolism pathway was quantified. Of these 44 genes analyzed, transcription of 6 genes were upregulated and 9 genes were downregulated. Earlier studies revealed that the transcription of genes for key enzymes like thymidylate synthase, thymidinekinase, and dihydropyrimidine dehydrogenase are regulated by 5-FU. This study identified some novel genes like thioredoxin reductase, ectonucleotide triphosphate dephosphorylase, and CTP synthase are regulated by 5-FU. The data also reveal large-scale perturbation in transcription of genes not involved directly in the known mechanism of action of 5-FU.

Item Type:	Journal Article
Publication:	Cancer investigation
Publisher:	Taylor and Francis Group
Additional Information:	Copyright for this article belongs to Taylor and Francis Group.
Keywords:	5-Fluorouracil; Microarray; Semiquantitative RT-PCR; Thioredoxin reductase; Thymidine kinase; Thymidylate synthase
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	19 Jul 2009 06:54
Last Modified:	27 Apr 2017 09:04
URI:	http://eprints.iisc.ac.in/id/eprint/20851

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