Sudha, S and Kumari, U and Rao, VS and Rao, AJ (1997) Identification of actin as an estradiol 17-beta-stimulated protein in the human placenta. In: Biochemistry And Molecular Biology International, 43 (5). pp. 955-966.
Full text not available from this repository. (Request a copy)Abstract
Addition of estradiol 17-beta to first trimester human placental minces resulted in an increased synthesis of a protein of apparent molecular weight 45 kDa. The specific involvement of estrogen in the stimulation of this protein was established by demonstrating a reduction in the level of this protein by the addition of CCS 16949 A, an inhibitor of aromatase, a key enzyme in the biosynthesis of estradiol 17-beta and ICI 182,780, an estrogen receptor antagonist. The protein was purified to homogeneity and N-terminal sequencing of two of the internal peptides obtained by enzymatic digestion of the protein, as well as the absence of a free N-terminal indicated that it could be actin. This was confirmed by Western blotting using commercially available actin antiserum. The role of estradiol 17-beta in the stimulation of actin synthesis in human placenta was also established by monitoring the quantitative inhibition of DNase I by actin.
| Item Type: | Journal Article |
|---|---|
| Publication: | Biochemistry And Molecular Biology International |
| Publisher: | Taylor and Francis Group |
| Additional Information: | Copyright of this article belongs Taylor and Francis Group. |
| Keywords: | Actin;cytoskeleton;DNase I;estradiol 17-beta;placenta;pregnancy. |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 17 Aug 2009 06:52 |
| Last Modified: | 17 Aug 2009 06:52 |
| URI: | http://eprints.iisc.ac.in/id/eprint/19378 |
Actions (login required)
 |
View Item |
