Hema, M and Reddy, Ch V Subba and Savithri, HS and Sreenivasulu, P (2008) Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles. In: Indian Journal Of Experimental Biology, 46 (11). pp. 793-796.
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Abstract
Coat protein (CP) gene of sugarcane streak mosaic virus-AP isolate (SCSMV-AP) was expressed in E. coli and recombinant CP (SCSMV-AP rCP) was purified by linear sucrose density gradient centrifugation. Observation of purified SCSMV-AP rCP under electron microscope revealed the presence of potyvirus-like particles (PVLPs). The assembled particles were shown to encapsidate CP gene transcripts by slot-blot hybridization.
| Item Type: | Journal Article |
|---|---|
| Publication: | Indian Journal Of Experimental Biology |
| Publisher: | National Institute of Science Communication and Information Resource |
| Additional Information: | Copyright of this article belongs to National Institute of Science Communication and Information Resource. |
| Keywords: | Potyvirus-like particles;Recombinant coat protein;Sugarcane streak mosaic virus. |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 22 Apr 2009 05:26 |
| Last Modified: | 22 Apr 2009 05:26 |
| URI: | http://eprints.iisc.ac.in/id/eprint/17673 |
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