Induction of classical and nonclassical MHC-I on mouse brain astrocytes by Japanese encephalitis virus

Abraham, Sojan and Manjunath, Ramanathapuram (2006) Induction of classical and nonclassical MHC-I on mouse brain astrocytes by Japanese encephalitis virus. In: Virus Research, 119 (2). pp. 216-220.

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Abstract

Infection with Flaviviruses upregulates the cell surface expression of MHC-I,MHC-II, ICAM- I (CD54),VCAM- I (CD106) and TAP proteins. Although all these studies have been confirmed using West Nile virus and other Flaviviruses, there are few reports that have examined the effects of Japanese encephalitis virus (JEV) infection directly on nonclassical and classical MHC expression in astrocytes. We show in this report that JEV infection of mouse brain astrocytes results in induction of the nonclassical MHC Class Ib genes, H-2T23, H-2Q4 and H-2T10 in addition to MHC-I, Type I (alpha,beta) IFNs, TAP-1, TAP-2, Tapasin, LMP-2, LMP-7 and LMP-10 but not IFN gamma, CD80, CD86 and MHC-II genes. The increased cel I surface expression of these antigens as well as induction of the genes mentioned above as measured by RT-PCR suggests that JEV infection may lead to the induction of classical MHC Class la as well as nonclassical MHC Class lb molecules.

Item Type:	Journal Article
Publication:	Virus Research
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Astrocyte;Nonclassical MHC-I;JEV.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	30 Mar 2009 09:41
Last Modified:	23 Feb 2012 05:52
URI:	http://eprints.iisc.ac.in/id/eprint/17430

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