Cloning and characterization of bonnet monkey GnRH receptor

Santra, Sumana and Rao, Veena S and Shanker, Gopi Y and Rao, Jagannadha A (2000) Cloning and characterization of bonnet monkey GnRH receptor. In: Molecular Human Reproduction, 6 (5). pp. 415-421.

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Abstract

Gonadotrophin releasing hormone (GnRH) plays an important role in the reproductive processes of both males and females. It is synthesized by the hypothalamus and binds to a specific receptor on the pituitary to bring about the release of the gonadotrophins, lutineizing hormone and follicle stimulating hormone, which in turn bring about the release of the gonadal steroids. Although the structure of the GnRH receptor (GnRHR) has been elucidated from a number of sources, no information is available about the receptor from the non-human primate species. Here we report the cloning and characterization of the receptor from the pituitary of the bonnet monkey. Antiserum to a bacterially expressed recombinant fragment was used in Western blot analysis and fluorescence microscopy to demonstrate the presence of GnRHR in both human and monkey placentae and pituitary.

Item Type:	Journal Article
Publication:	Molecular Human Reproduction
Publisher:	European Society of Human Reproduction and Embryology
Additional Information:	Copyright of this article belongs to European Society of Human Reproduction and Embryology
Keywords:	antibody;cloning;gonadotrophin releasing hormone receptor;monkey;pituitary
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	06 Aug 2008
Last Modified:	19 Sep 2010 04:48
URI:	http://eprints.iisc.ac.in/id/eprint/15454

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