Chandrashekaran, Siddamadappa and Shankar, Aparna Bhavani and Babu, Padmanabhan and Paul, Bindu Diana and Nagaraja, Valakunja (1999) Identification and characterization of a type II restriction endonuclease, Stri from streptomyces thermodiastaticus. In: Current Science, 77 (2). pp. 273-276.
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Abstract
A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5-C down arrow TCGAG-3 as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.
Item Type: | Journal Article |
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Publication: | Current Science |
Publisher: | Indian Academy of Sciences |
Additional Information: | The copyright belongs to Indian Academy of Sciences. |
Keywords: | endonuclease;streptomyces thermodiastaticus;enzyme |
Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
Date Deposited: | 07 Mar 2005 |
Last Modified: | 19 Sep 2010 04:15 |
URI: | http://eprints.iisc.ac.in/id/eprint/1541 |
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